STXBP1 Monoclonal antibody
STXBP1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF-P, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
1B5B3
验证数据展示
经过测试的应用
Positive WB detected in | fetal human brain tissue, Y79 cells, pig brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue |
Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
67137-1-Ig targets STXBP1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human |
Immunogen | STXBP1 fusion protein Ag28605 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | syntaxin binding protein 1 |
Synonyms | N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4 |
Calculated Molecular Weight | 594 aa, 68 kDa |
Observed Molecular Weight | 68 kDa |
GenBank Accession Number | BC015749 |
Gene Symbol | STXBP1 |
Gene ID (NCBI) | 6812 |
RRID | AB_2882436 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P61764 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for STXBP1 antibody 67137-1-Ig | Download protocol |
IHC protocol for STXBP1 antibody 67137-1-Ig | Download protocol |
IF protocol for STXBP1 antibody 67137-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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