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验证数据展示

  • IHC staining of mouse brain using Biotin-67137

    IHC staining of mouse brain using Biotin-67137

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-67137 (STXBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STXBP1 antibody included
  • IHC staining of mouse brain using Biotin-67137

    IHC staining of mouse brain using Biotin-67137

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-67137 (STXBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STXBP1 antibody included

Biotin Plus-conjugated STXBP1 Monoclonal antibody

STXBP1 Monoclonal Antibody for IHC
Cat No. Biotin-67137
CloneNo. 1B5B3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IHC

Synonyms

N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin Plus
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STXBP1 Ready-To-Use IHC Kit. STXBP1 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-67137 targets STXBP1 in IHC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IHC Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen STXBP1 fusion protein Ag28605 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name syntaxin binding protein 1
Synonyms N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4
Calculated Molecular Weight 594 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC015749
Gene Symbol STXBP1
Gene ID (NCBI) 6812
Conjugate Biotin Plus
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61764
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions.

实验方案

Product Specific Protocols
IHC protocol for Biotin Plus STXBP1 antibody Biotin-67137Download protocol
Standard Protocols
Click here to view our Standard Protocols