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验证数据展示

  • FC experiment of HeLa using CL594-67137

    FC experiment of HeLa using CL594-67137

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human STXBP1 (CL594-67137, Clone:1B5B3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated STXBP1 Monoclonal antibody

STXBP1 Monoclonal Antibody for FC (Intra)
Cat No. CL594-67137

Print datasheet

CloneNo. 1B5B3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Synonyms

N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STXBP1 Ready-To-Use IHC Kit. STXBP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67137-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67137 targets STXBP1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen STXBP1 fusion protein Ag28605 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name syntaxin binding protein 1
Synonyms N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4
Calculated Molecular Weight 594 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC015749
Gene Symbol STXBP1
Gene ID (NCBI) 6812
RRIDAB_3084749
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61764
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions.

实验方案

Product Specific Protocols
FC protocol for CL594 STXBP1 antibody CL594-67137Download protocol
Standard Protocols
Click here to view our Standard Protocols