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验证数据展示

  • WB analysis using CL488-67137

    WB analysis using CL488-67137

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67137 (STXBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL488-67137

    FC experiment of HeLa using CL488-67137

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human STXBP1 (CL488-67137, Clone:1B5B3) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated STXBP1 Monoclonal antibody

STXBP1 Monoclonal Antibody for WB, FC (Intra)
Cat No. CL488-67137

Print datasheet

CloneNo. 1B5B3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Synonyms

N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STXBP1 Ready-To-Use IHC Kit. STXBP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67137-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67137 targets STXBP1 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen STXBP1 fusion protein Ag28605 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name syntaxin binding protein 1
Synonyms N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4
Calculated Molecular Weight 594 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC015749
Gene Symbol STXBP1
Gene ID (NCBI) 6812
RRIDAB_3084312
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61764
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 STXBP1 antibody CL488-67137Download protocol
FC protocol for CL Plus 488 STXBP1 antibody CL488-67137Download protocol
Standard Protocols
Click here to view our Standard Protocols