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验证数据展示

  • WB analysis using CL488-67137

    WB analysis using CL488-67137

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67137 (STXBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL488-67137

    FC experiment of HeLa using CL488-67137

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human STXBP1 (CL488-67137, Clone:1B5B3) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated STXBP1 Monoclonal antibody

STXBP1 Monoclonal Antibody for WB, FC (Intra)
Cat No. CL488-67137

产品说明书

CloneNo. 1B5B3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, FC (Intra)

别名

N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STXBP1 Ready-To-Use IHC Kit. STXBP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67137-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67137 targets STXBP1 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 STXBP1 fusion protein Ag28605 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 syntaxin binding protein 1
别名 N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4
计算分子量 594 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC015749
基因名称 STXBP1
Gene ID (NCBI) 6812
RRIDAB_3084312
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61764
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 STXBP1 antibody CL488-67137Download protocol
FC protocol for CL Plus 488 STXBP1 antibody CL488-67137Download protocol
Standard Protocols
Click here to view our Standard Protocols