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验证数据展示

  • WB analysis of Y79 using Biotin-11459

    WB analysis of Y79 using Biotin-11459

    Y79 cells were subjected to SDS PAGE followed by western blot with Biotin-11459 (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using Biotin-11459

    IHC staining of mouse brain using Biotin-11459

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-11459 (STXBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STXBP1 antibody included
  • IHC staining of mouse brain using Biotin-11459

    IHC staining of mouse brain using Biotin-11459

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-11459 (STXBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STXBP1 antibody included
  • Featured Product
  • KD/KO验证

Biotin-conjugated STXBP1 Polyclonal antibody

STXBP1 Polyclonal Antibody for WB, IHC
Cat No. Biotin-11459

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC

别名

EIEE4, FLJ37475, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格: 

-/ -

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经过测试的应用

Positive WB detected inY79 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STXBP1 Ready-To-Use IHC Kit. STXBP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-11459 targets STXBP1 in WB, IHC applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC Application Description
经测试反应性 human, mouse, rat
免疫原 STXBP1 fusion protein Ag1978 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 syntaxin binding protein 1
别名 EIEE4, FLJ37475, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A
计算分子量 594 aa, 68 kDa
观测分子量 65-68 kDa
GenBank蛋白编号BC015749
基因名称 STXBP1
Gene ID (NCBI) 6812
RRIDAB_2918937
偶联类型 Biotin
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61764
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Biotin STXBP1 antibody Biotin-11459Download protocol
IHC protocol for Biotin STXBP1 antibody Biotin-11459Download protocol
Standard Protocols
Click here to view our Standard Protocols