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  • Featured Product
  • KD/KO Validated

SUFU Polyclonal antibody

SUFU Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 26759-1-AP

Print datasheet

Synonyms

UNQ650/PRO1280, Suppressor of fused homolog, SUFUH, PRO1280

验证数据展示

  • WB analysis using 26759-1-AP

    WB analysis using 26759-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26759-1-AP (SUFU antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 26759-1-AP

    IHC staining of human kidney using 26759-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26759-1-AP (SUFU Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with SUFU antibody included
  • IHC staining of human kidney using 26759-1-AP

    IHC staining of human kidney using 26759-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26759-1-AP (SUFU Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with SUFU antibody included
  • IP experiment of NIH/3T3 using 26759-1-AP

    IP experiment of NIH/3T3 using 26759-1-AP

    IP result of anti-SUFU (IP:26759-1-AP, 4ug; Detection:26759-1-AP 1:1000) with NIH/3T3 cells lysate 1600 ug.

  • WB analysis of HeLa using 26759-1-AP

    WB analysis of HeLa using 26759-1-AP

    WB result of SUFU antibody (26759-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUFU transfected HeLa cells.

  • IF Staining of HeLa using 26759-1-AP

    IF Staining of HeLa using 26759-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SUFU antibody (26759-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HEK-293 using 26759-1-AP

    FC experiment of HEK-293 using 26759-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug SUFU Polyclonal antibody (26759-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, C6 cells, HEK-293 cells, NIH/3T3 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy SUFU Ready-To-Use IHC Kit. SUFU primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26759-1-AP targets SUFU in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen SUFU fusion protein Ag25200 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name suppressor of fused homolog (Drosophila)
Synonyms UNQ650/PRO1280, Suppressor of fused homolog, SUFUH, PRO1280
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC013291
Gene Symbol SUFU
Gene ID (NCBI) 51684
RRIDAB_2880625
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UMX1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sufu (Suppressor of fused) is a central regulator of Hh (Hedgehog) signaling and acts as a tumor suppressor by maintaining the Gli (Glioma-associated oncogene homolog) transcription factors inactive. Loss of Sufu function promotes tumorigenesis.

实验方案

Product Specific Protocols
WB protocol for SUFU antibody 26759-1-APDownload protocol
IHC protocol for SUFU antibody 26759-1-APDownload protocol
IF protocol for SUFU antibody 26759-1-APDownload protocol
IP protocol for SUFU antibody 26759-1-APDownload protocol
FC protocol for SUFU antibody 26759-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Theranostics

Genetic and pharmacological activation of Hedgehog signaling inhibits osteoclastogenesis and attenuates titanium particle-induced osteolysis partly through suppressing the JNK/c-Fos-NFATc1 cascade.

Authors - Liwei Zhang
View Article
mouseWB

Phytother Res

Total flavonoid extract from Dracocephalum moldavica L. improves pulmonary fibrosis by reducing inflammation and inhibiting the hedgehog signaling pathway

Authors - Cheng-Hui He
View Article
humanWB

Phytother Res

Ursolic acid inhibits the activation of smoothened-independent non-canonical hedgehog pathway in colorectal cancer by suppressing AKT signaling cascade.

Authors - Li Chen
View Article
mouseIHC

Sci Rep

Suppression of Hedgehog signaling is required for cementum apposition.

Authors - Hwajung Choi
View Article
humanWB

Comput Intell Neurosci

Inhibitory Effect of Ursolic Acid on Proliferation and Migration of Renal Carcinoma Cells and Its Mechanism.

Authors - Xiao Lyu
View Article
humanIF

Cell Rep

The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling.

Authors - Leah Schembs
View Article