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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SUFU Polyclonal antibody

SUFU Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26759

Print datasheet

Synonyms

UNQ650/PRO1280, Suppressor of fused homolog, SUFUH, PRO1280

验证数据展示

  • IF Staining of HeLa using CL488-26759

    IF Staining of HeLa using CL488-26759

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 SUFU antibody (CL488-26759) at dilution of 1:200.

  • FC experiment of HEK-293 using CL488-26759

    FC experiment of HEK-293 using CL488-26759

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human SUFU (CL488-26759) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy SUFU Ready-To-Use IHC Kit. SUFU primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26759-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26759 targets SUFU in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen SUFU fusion protein Ag25200 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name suppressor of fused homolog (Drosophila)
Synonyms UNQ650/PRO1280, Suppressor of fused homolog, SUFUH, PRO1280
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC013291
Gene Symbol SUFU
Gene ID (NCBI) 51684
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UMX1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sufu (Suppressor of fused) is a central regulator of Hh (Hedgehog) signaling and acts as a tumor suppressor by maintaining the Gli (Glioma-associated oncogene homolog) transcription factors inactive. Loss of Sufu function promotes tumorigenesis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SUFU antibody CL488-26759Download protocol
FC protocol for CL Plus 488 SUFU antibody CL488-26759Download protocol
Standard Protocols
Click here to view our Standard Protocols