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CoraLite® Plus 488-conjugated SULT4A1 Monoclonal antibody

SULT4A1 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B9F6

Cat no : CL488-66106

Print datasheet

Synonyms

NST, hBR-STL-1, hBR-STL, EC:2.8.2.-, Brain sulfotransferase-like protein

验证数据展示

  • FC experiment of SH-SY5Y using CL488-66106

    FC experiment of SH-SY5Y using CL488-66106

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Sult4a1 Monoclonal Antibody (CL488-66106, Clone:1B9F6)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse brain using CL488-66106

    IF Staining of mouse brain using CL488-66106

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 SULT4A1 antibody (CL488-66106, Clone: 1B9F6 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 66106-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66106 targets SULT4A1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen SULT4A1 fusion protein Ag17962 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sulfotransferase family 4A, member 1
Synonyms NST, hBR-STL-1, hBR-STL, EC:2.8.2.-, Brain sulfotransferase-like protein
Calculated Molecular Weight 284 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC022459
Gene Symbol SULT4A1
Gene ID (NCBI) 25830
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BR01
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SULT4A1, also named as SULTX3, NST, ST4A1, hBR-STL and hBR-STL-1, belongs to the sulfotransferase 1 family. It may catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. SULT4A1 displays activity towards L-triiodothyronine, thyroxine, estrone, p-nitrophenol, 2-naphtylamine, and 2-naphtol. It may have a role in the sulfation of drugs and neurotransmitters in the CNS. SULT4A1 is a brain-specific sulfotransferase believed to be involved in the metabolism of neurotransmitters. It is a novel cytosolic sulfotransferase that is primarily expressed in the brain.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SULT4A1 antibody CL488-66106Download protocol
Standard Protocols
Click here to view our Standard Protocols