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验证数据展示

  • IF Staining of A549 using CL488-10329

    IF Staining of A549 using CL488-10329

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 SUMO1 antibody (CL488-10329) at dilution of 1:400.

  • FC experiment of A549 using CL488-10329

    FC experiment of A549 using CL488-10329

    1X10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SUMO1 (CL488-10329) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

CoraLite® Plus 488-conjugated SUMO1 Polyclonal antibody

SUMO1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10329

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

Small ubiquitin-related modifier 1, Small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy SUMO1 Ready-To-Use IHC Kit. SUMO1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10329-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10329 targets SUMO1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen SUMO1 fusion protein Ag0414 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
Synonyms Small ubiquitin-related modifier 1, Small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 10-12 kDa, 80-90 kDa
GenBank Accession NumberBC006462
Gene Symbol SUMO1
Gene ID (NCBI) 7341
RRIDAB_3083871
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP63165
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Small ubiquitin-related modifier 1 (SUMO1) belongs to the ubiquitin family and the SUMO subfamily as it contains 1 ubiquitin-like domain and is also 18% identical to ubiquitin (PMID: 9654451).

1. What is the molecular weight of SUMO1?

The molecular weight of SUMO1 is 12 kDa.

2. What is the cellular localization of SUMO1?

SUMO1 is located in the nuclear membrane and is recruited by BCL11A to the nuclear body (PMID: 18681895).

3. What modifications is SUMO1 subjected to?

SUMO1 function occurs after the cleavage of its precursor form by SENP1 or SENP2 (PMID: 27576863). Polymeric SUMO1 chains also undergo polyubiquitination by RNF4 (PMID: 18408734).

4. What is the function of SUMO1?

SUMO1 post-translationally modifies many proteins that have roles in an array of processes including transcriptional regulation, chromatin structure, and DNA repair. SUMO1 can covalently attach itself to protein as a monomer or a lysine-linked polymer via an isopeptide bond (PMID:11124955). This SUMO modification can regulate the localization and activity of the affected proteins.

5. What is SUMO1's involvement in disease?

Defects in SUMO1 as a result of a chromosomal translocation are associated with non-syndromic orofacial cleft type 10, or non-syndromic cleft lip, and are associated with cleft palate in two-thirds of cases (PMID: 18983974).


实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SUMO1 antibody CL488-10329Download protocol
Standard Protocols
Click here to view our Standard Protocols