验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HSC-T6 cells, HeLa cells, Jurkat cells, L02 cells, PC-12 cells |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11251-1-AP targets SUMO2/3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SUMO2/3 fusion protein Ag1778 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) |
| 别名 | SUMO2, SMT3B, Small ubiquitin-related modifier 2, Sentrin-2, Sentrin2 |
| 计算分子量 | 11 kDa |
| 观测分子量 | 11-20 kDa |
| GenBank蛋白编号 | BC016775 |
| 基因名称 | SUMO2 |
| Gene ID (NCBI) | 6613 |
| RRID | AB_2198405 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P61956 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SUMO2/3 antibody 11251-1-AP | Download protocol |
| IHC protocol for SUMO2/3 antibody 11251-1-AP | Download protocol |
| IF protocol for SUMO2/3 antibody 11251-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility | ||
Theranostics Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation. | ||
mSystems Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia. | ||
iScience Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress | ||
Int J Mol Med SUMO2 modification of Aurora B and its impact on follicular development and atresia in the mouse ovary. | ||
Int J Oncol Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9 |
