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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SUMO2/3 Polyclonal antibody

SUMO2/3 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11251

Print datasheet

Synonyms

HSMT3, Sentrin 2, SMT3 homolog 2, SMT3B, SMT3H2, SUMO 2, SUMO 3, SUMO2, Ubiquitin like protein SMT3B

验证数据展示

  • IF Staining of HEK-293 using CL488-11251

    IF Staining of HEK-293 using CL488-11251

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 SUMO2/3 antibody (CL488-11251) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 11251-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11251 targets SUMO2/3 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen SUMO2/3 fusion protein Ag1778 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
Synonyms HSMT3, Sentrin 2, SMT3 homolog 2, SMT3B, SMT3H2, SUMO 2, SUMO 3, SUMO2, Ubiquitin like protein SMT3B
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 11-20 kDa
GenBank Accession NumberBC016775
Gene Symbol SUMO2
Gene ID (NCBI) 6613
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen Affinity Purified
UNIPROT IDP61956
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SUMO2/3 antibody CL488-11251Download protocol
Standard Protocols
Click here to view our Standard Protocols