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验证数据展示

  • WB analysis using 27556-1-AP (same clone as 27556-1-PBS)

    WB analysis using 27556-1-AP (same clone as 27556-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27556-1-AP (SUN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27556-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 27556-1-AP (same clone as 27556-1-PBS)

    WB analysis of HeLa using 27556-1-AP (same clone as 27556-1-PBS)

    WB result of SUN2 antibody (27556-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUN2 transfected HeLa cells. This data was developed using the same antibody clone with 27556-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 27556-1-AP (same clone as 27556-1-PBS)

    IHC staining of human stomach using 27556-1-AP (same clone as 27556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27556-1-AP (SUN2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27556-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 27556-1-AP (same clone as 27556-1-PBS)

    IF Staining of HeLa using 27556-1-AP (same clone as 27556-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUN2 antibody (27556-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27556-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SUN2 Polyclonal antibody, PBS Only

SUN2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27556-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

UNC84B, FRIGG, KIAA0668, Protein unc-84 homolog B, Rab5-interacting protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

27556-1-PBS targets SUN2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 SUN2 fusion protein Ag16077 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 unc-84 homolog B (C. elegans)
别名 UNC84B, FRIGG, KIAA0668, Protein unc-84 homolog B, Rab5-interacting protein
计算分子量 717 aa, 80 kDa
观测分子量 80-85 kDa
GenBank蛋白编号BC111717
基因名称 SUN2
Gene ID (NCBI) 25777
RRIDAB_2880906
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UH99
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).