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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83052-6-RR (same clone as 83052-6-PBS)

    WB analysis using 83052-6-RR (same clone as 83052-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83052-6-PBS in a different storage buffer formulation.

  • WB analysis using 83052-6-RR (same clone as 83052-6-PBS)

    WB analysis using 83052-6-RR (same clone as 83052-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83052-6-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 83052-6-RR (same clone as 83052-6-PBS)

    WB analysis of MCF-7 using 83052-6-RR (same clone as 83052-6-PBS)

    WB result of SURF1 antibody (83052-6-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SURF1 transfected MCF-7 cells. This data was developed using the same antibody clone with 83052-6-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83052-6-RR (same clone as 83052-6-PBS)

    FC experiment of HepG2 using 83052-6-RR (same clone as 83052-6-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SURF1 (83052-6-RR, Clone:230210E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83052-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83052-6-RR

    Affinity and Kinetic Characterization of 83052-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83052-6-RR against Human SURF1 were performed. The affinity constant is 0.509 nM.

  • Featured Product
  • KD/KO Validated

SURF1 Recombinant antibody, PBS Only

SURF1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 83052-6-PBS

Print datasheet

CloneNo. 230210E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210E12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

83052-6-PBS targets SURF1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SURF1 fusion protein Ag3959 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name surfeit 1
Synonyms Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210E12
Calculated Molecular Weight 33 kDa
Observed Molecular Weight30-31 kDa
GenBank Accession NumberBC028314
Gene Symbol SURF1
Gene ID (NCBI) 6834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15526
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C