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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83052-6-RR (same clone as 83052-6-PBS)

    WB analysis using 83052-6-RR (same clone as 83052-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83052-6-PBS in a different storage buffer formulation.

  • WB analysis using 83052-6-RR (same clone as 83052-6-PBS)

    WB analysis using 83052-6-RR (same clone as 83052-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83052-6-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 83052-6-RR (same clone as 83052-6-PBS)

    WB analysis of MCF-7 using 83052-6-RR (same clone as 83052-6-PBS)

    WB result of SURF1 antibody (83052-6-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SURF1 transfected MCF-7 cells. This data was developed using the same antibody clone with 83052-6-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83052-6-RR (same clone as 83052-6-PBS)

    FC experiment of HepG2 using 83052-6-RR (same clone as 83052-6-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SURF1 (83052-6-RR, Clone:230210E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83052-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83052-6-RR

    Affinity and Kinetic Characterization of 83052-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83052-6-RR against Human SURF1 were performed. The affinity constant is 0.509 nM.

  • Featured Product
  • KD/KO验证

SURF1 Recombinant antibody, PBS Only

SURF1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 83052-6-PBS

产品说明书

CloneNo. 230210E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Indirect ELISA

别名

Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210E12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83052-6-PBS targets SURF1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SURF1 fusion protein Ag3959 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 surfeit 1
别名 Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210E12
计算分子量 33 kDa
观测分子量30-31 kDa
GenBank蛋白编号BC028314
基因名称 SURF1
Gene ID (NCBI) 6834
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15526
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C