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Conjugate
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Conjugate
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Conjugate
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SURF1 Recombinant antibody

SURF1 Recombinant Antibody for WB,FC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB,FC,ELISA

Conjugate

Unconjugated

CloneNo.

230210E12

Cat No : 83052-6-RR

Print datasheet

Synonyms

SURF 1, SURF1, surfeit 1, Surfeit locus protein 1

验证数据展示

  • WB analysis using 83052-6-RR

    WB analysis using 83052-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83052-6-RR

    WB analysis using 83052-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83052-6-RR

    FC experiment of HepG2 using 83052-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SURF1 (83052-6-RR, Clone:230210E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue, MCF-7 cells, rat liver tissue, rat heart tissue
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83052-6-RR targets SURF1 in WB, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB,FC,ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen SURF1 fusion protein Ag3959 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name surfeit 1
Synonyms SURF 1, SURF1, surfeit 1, Surfeit locus protein 1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight30-31 kDa
GenBank Accession NumberBC028314
Gene Symbol SURF1
Gene ID (NCBI) 6834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15526
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SURF1 antibody 83052-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols