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验证数据展示

  • WB analysis using 83052-6-RR

    WB analysis using 83052-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83052-6-RR

    WB analysis using 83052-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 83052-6-RR

    WB analysis of MCF-7 using 83052-6-RR

    WB result of SURF1 antibody (83052-6-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SURF1 transfected MCF-7 cells.

  • FC experiment of HepG2 using 83052-6-RR

    FC experiment of HepG2 using 83052-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SURF1 (83052-6-RR, Clone:230210E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83052-6-RR

    Affinity and Kinetic Characterization of 83052-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83052-6-RR against Human SURF1 were performed. The affinity constant is 0.509 nM.

  • Featured Product
  • KD/KO验证

SURF1 Recombinant antibody

SURF1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83052-6-RR

产品说明书

CloneNo. 230210E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210E12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue, rat heart tissue
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83052-6-RR targets SURF1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SURF1 fusion protein Ag3959 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 surfeit 1
别名 Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210E12
计算分子量 33 kDa
观测分子量30-31 kDa
GenBank蛋白编号BC028314
基因名称 SURF1
Gene ID (NCBI) 6834
RRIDAB_3670776
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15526
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SURF1 antibody 83052-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols