验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83052-6 targets SURF1 in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 | SURF1 fusion protein Ag3959 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | surfeit 1 |
| 别名 | Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210E12 |
| 计算分子量 | 33 kDa |
| 观测分子量 | 30-31 kDa |
| GenBank蛋白编号 | BC028314 |
| 基因名称 | SURF1 |
| Gene ID (NCBI) | 6834 |
| RRID | AB_3673175 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15526 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SURF1 (Surfeit locus protein 1), also known as SURF-1. It is expected to be located in mitochondrion inner membrane, which is ubiquitinated in thyroid and kidney. This gene encodes a protein localized to the inner mitochondrial membrane and thought to be involved in the biogenesis of the cytochrome c oxidase complex. The protein is a member of the SURF1 family, which includes the related yeast protein SHY1 and rickettsial protein RP733. The gene is located in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity, where it shares a bidirectional promoter with SURF2 on the opposite strand. Defects in this gene are a cause of Leigh syndrome, a severe neurological disorder that is commonly associated with systemic cytochrome c oxidase deficiency. The molecular weight of SURF1 is 33 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 SURF1 antibody CL488-83052-6 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |