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CoraLite® Plus 488-conjugated SURF1 Recombinant antibody

SURF1 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230210E12

Cat No : CL488-83052-6

Print datasheet

Synonyms

Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210E12

验证数据展示

  • FC experiment of HepG2 using CL488-83052-6

    FC experiment of HepG2 using CL488-83052-6

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Surf1 Recombinant Antibody (CL488-83052-6, Clone:230210E12)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 83052-6-PBS

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83052-6 targets SURF1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen SURF1 fusion protein Ag3959 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name surfeit 1
Synonyms Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210E12
Calculated Molecular Weight 33 kDa
Observed Molecular Weight30-31 kDa
GenBank Accession NumberBC028314
Gene Symbol SURF1
Gene ID (NCBI) 6834
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15526
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SURF1 (Surfeit locus protein 1), also known as SURF-1. It is expected to be located in mitochondrion inner membrane, which is ubiquitinated in thyroid and kidney. This gene encodes a protein localized to the inner mitochondrial membrane and thought to be involved in the biogenesis of the cytochrome c oxidase complex. The protein is a member of the SURF1 family, which includes the related yeast protein SHY1 and rickettsial protein RP733. The gene is located in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity, where it shares a bidirectional promoter with SURF2 on the opposite strand. Defects in this gene are a cause of Leigh syndrome, a severe neurological disorder that is commonly associated with systemic cytochrome c oxidase deficiency. The molecular weight of SURF1 is 33 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SURF1 antibody CL488-83052-6Download protocol
Standard Protocols
Click here to view our Standard Protocols