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验证数据展示

  • WB analysis using 30711-1-AP

    WB analysis using 30711-1-AP

    SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 30711-1-AP (SUSD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30711-1-AP

    WB analysis using 30711-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30711-1-AP (SUSD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 30711-1-AP

    IHC staining of mouse kidney using 30711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30711-1-AP (SUSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SUSD2 Polyclonal antibody

SUSD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30711-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

sushi domain containing 2, BK65A6.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSK-BR-3 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30711-1-AP targets SUSD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SUSD2 fusion protein Ag33630 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sushi domain containing 2
别名 sushi domain containing 2, BK65A6.2
计算分子量90 kDa
观测分子量60 kDa, 90 kDa, 110 kDa
GenBank蛋白编号NM_019601
基因名称 SUSD2
Gene ID (NCBI) 56241
RRIDAB_3669753
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UGT4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sushi domain containing 2 (SUSD2) is a type I transmembrane protein that contains a somatomedin B, AMOP, von Willebrand factor type D and Sushi domains, which are frequently found in molecules associated with cell-cell and cell-matrix adhesion. SUSD2 is composed of 822 amino acids with a predicted molecular weight of 90.4 kDa (PMID: 31387209). Three specific SUSD2 bands were present, the glycosylated, full-length protein (~110 kDa), ~90 kDa band represents full-length SUSD2 with minimal glycosylation, and a strong ~60 kDa band, represents a cleaved product of SUSD2 at the GD-PH sequence in the endoplasmic reticulum (PMID:27775699, 32595828).

实验方案

Product Specific Protocols
WB protocol for SUSD2 antibody 30711-1-APDownload protocol
IHC protocol for SUSD2 antibody 30711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols