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验证数据展示

  • IF Staining of HepG2 using CL488-16272

    IF Staining of HepG2 using CL488-16272

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SYAP1 antibody (CL488-16272) at dilution of 1:200, CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated SYAP1 Polyclonal antibody

SYAP1 Polyclonal Antibody for IF/ICC
Cat No. CL488-16272

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

DKFZp686K221, FLJ14495, FLJ44185, PRO3113, SYAP1, Synapse associated protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 16272-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16272 targets SYAP1 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 SYAP1 fusion protein Ag9342 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synapse associated protein 1, SAP47 homolog (Drosophila)
别名 DKFZp686K221, FLJ14495, FLJ44185, PRO3113, SYAP1, Synapse associated protein 1
计算分子量 352 aa, 40 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC014657
基因名称 SYAP1
Gene ID (NCBI) 94056
RRIDAB_3672647
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96A49
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SYAP1 antibody CL488-16272Download protocol
Standard Protocols
Click here to view our Standard Protocols