SCP3 Polyclonal antibody
SCP3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, HepG2 cells, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | Jurkat cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23024-1-AP targets SCP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | SCP3 fusion protein Ag19330 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | synaptonemal complex protein 3 |
| Synonyms | SYCP3, Synaptonemal complex protein 3, SCP-3, SCP 3, COR1 |
| Calculated Molecular Weight | 236 aa, 28 kDa |
| Observed Molecular Weight | 30 kDa, 33 kDa |
| GenBank Accession Number | BC062662 |
| Gene Symbol | SYCP3 |
| Gene ID (NCBI) | 50511 |
| RRID | AB_11232426 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IZU3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SYCP3 is an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. SYCP3 is required for centromere pairing during meiosis in male germ cells. SYCP3 is also required for normal meiosis during spermatogenesis and male fertility. Mutations in SYCP3 are associated with azoospermia in males and susceptibility to pregnancy loss in females.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SCP3 antibody 23024-1-AP | Download protocol |
| IF protocol for SCP3 antibody 23024-1-AP | Download protocol |
| IP protocol for SCP3 antibody 23024-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination. | ||
Cell Death Differ NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis. | ||
Int J Nanomedicine Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway. | ||
Biomed Pharmacother Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway. | ||
Andrology Exposure to elevated temperature affects the expression of piwi-interacting RNAs and associated transcripts in mouse testes |
