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验证数据展示

  • FC experiment of Jurkat using CL488-23024

    FC experiment of Jurkat using CL488-23024

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human SCP3 (CL488-23024) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated SCP3 Polyclonal antibody

SCP3 Polyclonal Antibody for FC (Intra)
Cat No. CL488-23024

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

SYCP3, Synaptonemal complex protein 3, SCP-3, SCP 3, COR1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488

Size: 
100 μL

¥2800/ 100 μL

浓度: 1000 μg/ml

库存：现货

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 23024-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-23024 targets SCP3 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen SCP3 fusion protein Ag19330 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptonemal complex protein 3
Synonyms SYCP3, Synaptonemal complex protein 3, SCP-3, SCP 3, COR1
Calculated Molecular Weight 236 aa, 28 kDa
Observed Molecular Weight30 kDa, 33 kDa
GenBank Accession NumberBC062662
Gene Symbol SYCP3
Gene ID (NCBI) 50511
RRIDAB_3084071
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZU3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYCP3 is an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. SYCP3 is required for centromere pairing during meiosis in male germ cells. SYCP3 is also required for normal meiosis during spermatogenesis and male fertility. Mutations in SYCP3 are associated with azoospermia in males and susceptibility to pregnancy loss in females.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SCP3 antibody CL488-23024Download protocol
Standard Protocols
Click here to view our Standard Protocols