SYK Recombinant antibody, PBS Only

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H24

Cat No : 83331-1-PBS

Synonyms

DKFZp313N1010, FLJ25043, FLJ37489, spleen tyrosine kinase, SYK, Tyrosine protein kinase SYK

WB analysis of Ramos using 83331-1-RR (same clone as 83331-1-PBS)
Non-treated Ramos cells, and pervanadate treated Ramos cells were subjected to SDS PAGE followed by western blot with 83331-1-RR (Phospho-SYK (Tyr323) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 83331-1-PBS in a different storage buffer formulation.
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Non-treated Ramos cells, and pervanadate treated Ramos cells were subjected to SDS PAGE followed by western blot with 83331-1-RR (Phospho-SYK (Tyr323) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 83331-1-PBS in a different storage buffer formulation.
IF Staining of Ramos using 83331-1-RR (same clone as 83331-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed pervanadate treated Ramos cells using Phospho-SYK (Tyr323) antibody (83331-1-RR, Clone: 2H24 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83331-1-PBS in a different storage buffer formulation.

Size

83331-1-PBS targets SYK in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications	WB, IF, Indirect ELISA Application Description
Tested Reactivity	Human
Immunogen	Peptide 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Full Name	spleen tyrosine kinase
Synonyms	DKFZp313N1010, FLJ25043, FLJ37489, spleen tyrosine kinase, SYK, Tyrosine protein kinase SYK
Calculated Molecular Weight	72 kDa
GenBank Accession Number	BC001645
Gene Symbol	SYK
Gene ID (NCBI)	6850
ENSEMBL Gene ID	ENSG00000165025
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	P43405
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.