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验证数据展示

  • WB analysis of Ramos using 83331-1-RR

    WB analysis of Ramos using 83331-1-RR

    Non-treated Ramos cells, and pervanadate treated Ramos cells were subjected to SDS PAGE followed by western blot with 83331-1-RR (Phospho-SYK (Tyr323) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control.

  • IF Staining of Ramos using 83331-1-RR

    IF Staining of Ramos using 83331-1-RR

    Immunofluorescent analysis of (4% PFA) fixed pervanadate treated Ramos cells using Phospho-SYK (Tyr323) antibody (83331-1-RR, Clone: 2H24 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-SYK (Tyr323) Recombinant antibody

Phospho-SYK (Tyr323) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83331-1-RR

产品说明书

CloneNo. 2H24

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

DKFZp313N1010, FLJ25043, FLJ37489, Phospho-SYK (Tyr323), spleen tyrosine kinase, SYK, Tyrosine protein kinase SYK

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inpervanadate treated Ramos cells
Positive IF/ICC detected inpervanadate treated Ramos cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83331-1-RR targets Phospho-SYK (Tyr323) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 spleen tyrosine kinase
别名 DKFZp313N1010, FLJ25043, FLJ37489, Phospho-SYK (Tyr323), spleen tyrosine kinase, SYK, Tyrosine protein kinase SYK
计算分子量 72 kDa
观测分子量70 kDa
GenBank蛋白编号BC001645
基因名称 SYK
Gene ID (NCBI) 6850
ENSEMBL Gene IDENSG00000165025
RRIDAB_3670995
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP43405
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. The study found that in human platelets, inhibition of PKC leads to Syk hyperphosphorylation on residues Tyr-525/526 whereas Tyr-323 and Tyr-352 phosphorylations are unaffected. PKC negatively regulates Syk activity, as evidenced by hyperphosphorylation of downstream targets, LAT and PLCγ2 upon PKC inhibition. (PMID: 23960082)

实验方案

Product Specific Protocols
WB protocol for Phospho-SYK (Tyr323) antibody 83331-1-RRDownload protocol
IF protocol for Phospho-SYK (Tyr323) antibody 83331-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols