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Phospho-SYK (Tyr323) Recombinant antibody

Phospho-SYK (Tyr323) Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H24

Cat No : 83331-1-RR

Print datasheet

Synonyms

DKFZp313N1010, FLJ25043, FLJ37489, Phospho-SYK (Tyr323), spleen tyrosine kinase, SYK, Tyrosine protein kinase SYK

验证数据展示

  • WB analysis of Ramos using 83331-1-RR

    WB analysis of Ramos using 83331-1-RR

    Non-treated Ramos cells, and pervanadate treated Ramos cells were subjected to SDS PAGE followed by western blot with 83331-1-RR (Phospho-SYK (Tyr323) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control.

  • IF Staining of Ramos using 83331-1-RR

    IF Staining of Ramos using 83331-1-RR

    Immunofluorescent analysis of (4% PFA) fixed pervanadate treated Ramos cells using Phospho-SYK (Tyr323) antibody (83331-1-RR, Clone: 2H24 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inpervanadate treated Ramos cells
Positive IF detected inpervanadate treated Ramos cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83331-1-RR targets Phospho-SYK (Tyr323) in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name spleen tyrosine kinase
Synonyms DKFZp313N1010, FLJ25043, FLJ37489, Phospho-SYK (Tyr323), spleen tyrosine kinase, SYK, Tyrosine protein kinase SYK
Calculated Molecular Weight 72 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC001645
Gene Symbol SYK
Gene ID (NCBI) 6850
ENSEMBL Gene IDENSG00000165025
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP43405
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. The study found that in human platelets, inhibition of PKC leads to Syk hyperphosphorylation on residues Tyr-525/526 whereas Tyr-323 and Tyr-352 phosphorylations are unaffected. PKC negatively regulates Syk activity, as evidenced by hyperphosphorylation of downstream targets, LAT and PLCγ2 upon PKC inhibition. (PMID: 23960082)

实验方案

Product Specific Protocols
WB protocol for Phospho-SYK (Tyr323) antibody 83331-1-RRDownload protocol
IF protocol for Phospho-SYK (Tyr323) antibody 83331-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols