验证数据展示
经过测试的应用
| Positive IF/ICC detected in | pervanadate treated Ramos cells |
For other applications, we recommend the unconjugated version of this antibody, 83331-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83331 targets Phospho-SYK (Tyr323) in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | spleen tyrosine kinase |
| 别名 | p SYK Tyr323, p SYK (Tyr323), p SYK, Phospho SYK, Phospho SYK (Tyr323) |
| 计算分子量 | 72 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC001645 |
| 基因名称 | SYK |
| Gene ID (NCBI) | 6850 |
| ENSEMBL Gene ID | ENSG00000165025 |
| RRID | AB_3673241 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P43405 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. The study found that in human platelets, inhibition of PKC leads to Syk hyperphosphorylation on residues Tyr-525/526 whereas Tyr-323 and Tyr-352 phosphorylations are unaffected. PKC negatively regulates Syk activity, as evidenced by hyperphosphorylation of downstream targets, LAT and PLCγ2 upon PKC inhibition. (PMID: 23960082)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Phospho-SYK (Tyr323) antibody CL488-83331 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |