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Syncoilin Polyclonal antibody

Syncoilin Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25151-1-AP

Print datasheet

Synonyms

SYNC, SYNC1, SYNCOILIN, Syncoilin 1

验证数据展示

  • IP experiment of mouse heart using 25151-1-AP

    IP experiment of mouse heart using 25151-1-AP

    IP result of anti-Syncoilin (IP:25151-1-AP, 4ug; Detection:25151-1-AP 1:800) with mouse heart tissue lysate 4000ug.

  • IHC staining of human skeletal muscle using 25151-1-AP

    IHC staining of human skeletal muscle using 25151-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25151-1-AP (SYNC Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 25151-1-AP

    IHC staining of human skeletal muscle using 25151-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25151-1-AP (SYNC Antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis using 25151-1-AP

    WB analysis using 25151-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25151-1-AP (Syncoilin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 25151-1-AP

    IHC staining of mouse heart using 25151-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25151-1-AP (Syncoilin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 25151-1-AP

    IHC staining of mouse heart using 25151-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25151-1-AP (Syncoilin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 25151-1-AP

    IHC staining of mouse skeletal muscle using 25151-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25151-1-AP (Syncoilin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 25151-1-AP

    IHC staining of mouse skeletal muscle using 25151-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25151-1-AP (Syncoilin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse heart tissue, human heart tissue, mouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

25151-1-AP targets Syncoilin in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen Syncoilin fusion protein Ag18565 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name syncoilin, intermediate filament protein
Synonyms SYNC, SYNC1, SYNCOILIN, Syncoilin 1
Calculated Molecular Weight 482 aa, 55 kDa
Observed Molecular Weight 64-68 kDa, 55 kDa
GenBank Accession NumberBC119700
Gene Symbol SYNC
Gene ID (NCBI) 81493
RRIDAB_2879926
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H7C4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Syncoilin antibody 25151-1-APDownload protocol
IHC protocol for Syncoilin antibody 25151-1-APDownload protocol
IP protocol for Syncoilin antibody 25151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Invest Ophthalmol Vis Sci

The Cytoskeleton in the Extraocular Muscles of Desmin Knockout Mice.

Authors - Maria Angels Rodríguez
View Article