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验证数据展示

  • WB analysis of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

    WB analysis of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67339-1-Ig (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

    IHC staining of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

    IHC staining of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of human kidney using 67339-1-Ig (same clone as 67339-1-PBS)

    IHC staining of human kidney using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • IF Staining of mouse brain using 67339-1-Ig (same clone as 67339-1-PBS)

    IF Staining of mouse brain using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse kidney using 67339-1-Ig (same clone as 67339-1-PBS)

    IF Staining of mouse kidney using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 67339-1-Ig (same clone as 67339-1-PBS)

    IF Staining of HEK-293 using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

SYNPO Monoclonal antibody, PBS Only

SYNPO Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 67339-1-PBS

产品说明书

CloneNo. 1E1B1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

Synaptopodin, 1E1B1, KIAA1029, snaptopodin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SYNPO Ready-To-Use IHC Kit. SYNPO primary antibody included.

产品信息

67339-1-PBS targets SYNPO in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 SYNPO fusion protein Ag15089 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 synaptopodin
别名 Synaptopodin, 1E1B1, KIAA1029, snaptopodin
计算分子量 929 aa, 99 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC146665
基因名称 SYNPO
Gene ID (NCBI) 11346
RRIDAB_2882597
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8N3V7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)