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SYNPO Monoclonal antibody, PBS Only

SYNPO Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1E1B1

Cat no : 67339-1-PBS

Print datasheet

Synonyms

Synaptopodin, snaptopodin, KIAA1029, 1E1B1

验证数据展示

  • WB analysis of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

    WB analysis of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67339-1-Ig (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

    IHC staining of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

    IHC staining of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of human kidney using 67339-1-Ig (same clone as 67339-1-PBS)

    IHC staining of human kidney using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • IF Staining of mouse brain using 67339-1-Ig (same clone as 67339-1-PBS)

    IF Staining of mouse brain using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse kidney using 67339-1-Ig (same clone as 67339-1-PBS)

    IF Staining of mouse kidney using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 67339-1-Ig (same clone as 67339-1-PBS)

    IF Staining of HEK-293 using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SYNPO Ready-To-Use IHC Kit. SYNPO primary antibody included.

产品信息

67339-1-PBS targets SYNPO in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen SYNPO fusion protein Ag15089 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name synaptopodin
Synonyms Synaptopodin, snaptopodin, KIAA1029, 1E1B1
Calculated Molecular Weight 929 aa, 99 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC146665
Gene Symbol SYNPO
Gene ID (NCBI) 11346
RRIDAB_2882597
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N3V7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)