验证数据展示
经过测试的应用
Positive WB detected in | rat brain tissue, pig brain tissue, mouse brain tissue |
Positive IHC detected in | rat brain tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue, mouse kidney tissue |
Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:750-1:3000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
67339-1-Ig targets SYNPO in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat, pig |
文献引用反应性 | mouse |
免疫原 | SYNPO fusion protein Ag15089 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | synaptopodin |
别名 | Synaptopodin, 1E1B1, KIAA1029, snaptopodin |
计算分子量 | 929 aa, 99 kDa |
观测分子量 | 100-110 kDa |
GenBank蛋白编号 | BC146665 |
基因名称 | SYNPO |
Gene ID (NCBI) | 11346 |
RRID | AB_2882597 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q8N3V7 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)
实验方案
Product Specific Protocols | |
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WB protocol for SYNPO antibody 67339-1-Ig | Download protocol |
IHC protocol for SYNPO antibody 67339-1-Ig | Download protocol |
IF protocol for SYNPO antibody 67339-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Cell Dev Biol Umbilical Cord Mesenchymal Stem Cells Ameliorate Kidney Injury in MRL/Ipr Mice Through the TGF-β1 Pathway. | ||
Cell Death Dis Sestrin2 remedies podocyte injury via orchestrating TSP-1/TGF-β1/Smad3 axis in diabetic kidney disease. |