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验证数据展示

  • IF Staining of mouse brain using CL488-67339

    IF Staining of mouse brain using CL488-67339

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 SYNPO antibody (CL488-67339, Clone: 1E1B1 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated SYNPO Monoclonal antibody

SYNPO Monoclonal Antibody for IF-P
Cat No. CL488-67339

Print datasheet

CloneNo. 1E1B1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

IF-P

Synonyms

KIAA1029, synaptopodin, SYNPO

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy SYNPO Ready-To-Use IHC Kit. SYNPO primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67339-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67339 targets SYNPO in IF-P applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen SYNPO fusion protein Ag15089 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name synaptopodin
Synonyms KIAA1029, synaptopodin, SYNPO
Calculated Molecular Weight 929 aa, 99 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC146665
Gene Symbol SYNPO
Gene ID (NCBI) 11346
RRIDAB_2934539
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N3V7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SYNPO antibody CL488-67339Download protocol
Standard Protocols
Click here to view our Standard Protocols