验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy SYNPO Ready-To-Use IHC Kit. SYNPO primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67339-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67339 targets SYNPO in IF-P applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 | SYNPO fusion protein Ag15089 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | synaptopodin |
| 别名 | Synaptopodin, 1E1B1, KIAA1029, snaptopodin |
| 计算分子量 | 929 aa, 99 kDa |
| 观测分子量 | 100-110 kDa |
| GenBank蛋白编号 | BC146665 |
| 基因名称 | SYNPO |
| Gene ID (NCBI) | 11346 |
| RRID | AB_2934539 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8N3V7 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SYNPO antibody CL488-67339 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |