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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Secretogranin III Polyclonal antibody

Secretogranin III Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-10954

Print datasheet

Synonyms

FLJ90833, SCG3, Secretogranin 3, secretogranin III, SGIII

验证数据展示

  • IF Staining of mouse pancreas using CL488-10954

    IF Staining of mouse pancreas using CL488-10954

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CoraLite® Plus 488 Secretogranin III antibody (CL488-10954) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF detected inmouse pancreas tissue
For other applications, we recommend the unconjugated version of this antibody, 10954-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10954 targets Secretogranin III in IF applications and shows reactivity with human, mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse
Immunogen Secretogranin III fusion protein Ag1397 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name secretogranin III
Synonyms FLJ90833, SCG3, Secretogranin 3, secretogranin III, SGIII
Calculated Molecular Weight 53 kDa
GenBank Accession NumberBC014539
Gene Symbol SCG3
Gene ID (NCBI) 29106
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXD2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Secretogranin III (SCG3), a member of the granin protein family, is expressed specifically in neuronal and endocrine cells. SCG3 was synthesized as a 61- or 63-kDa protein and processed to a 48-kDa form which, in turn, was partially cleaved to fragments of 28 and 20 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Secretogranin III antibody CL488-10954Download protocol
Standard Protocols
Click here to view our Standard Protocols