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  • Featured Product
  • KD/KO Validated

Securin Polyclonal antibody, PBS Only

Securin Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18040-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 18040-1-AP (same clone as 18040-1-PBS)

    WB analysis using 18040-1-AP (same clone as 18040-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18040-1-AP (Securin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18040-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 18040-1-AP (same clone as 18040-1-PBS)

    IHC staining of human urothelial carcinoma using 18040-1-AP (same clone as 18040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 18040-1-AP (Securin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18040-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTTG1/Securin antibody included
  • IF Staining of HepG2 using 18040-1-AP (same clone as 18040-1-PBS)

    IF Staining of HepG2 using 18040-1-AP (same clone as 18040-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Securin / PTTG1 antibody (18040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18040-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 18040-1-AP (same clone as 18040-1-PBS)

    IF Staining of U2OS using 18040-1-AP (same clone as 18040-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Securin antibody (18040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18040-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PTTG1/Securin Ready-To-Use IHC Kit. PTTG1/Securin primary antibody included.

产品信息

18040-1-PBS targets Securin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Securin fusion protein Ag12670 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pituitary tumor-transforming 1
Synonyms
Calculated Molecular Weight 202 aa, 22 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC101834
Gene Symbol Securin
Gene ID (NCBI) 9232
RRIDAB_2173410
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95997
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.