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Selenoprotein P Polyclonal antibody

Selenoprotein P Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30249-1-AP

Print datasheet

Synonyms

Selenoprotein P, selenoprotein P, plasma, 1, SELP, SeP, SEPP1

验证数据展示

  • WB analysis of human plasma using 30249-1-AP

    WB analysis of human plasma using 30249-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 30249-1-AP (Selenoprotein P antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 30249-1-AP

    IHC staining of human liver using 30249-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30249-1-AP (Selenoprotein P antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 30249-1-AP

    IF Staining of HepG2 using 30249-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Selenoprotein P antibody (30249-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30249-1-AP targets Selenoprotein P in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen Selenoprotein P fusion protein Ag32952 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name selenoprotein P, plasma, 1
Synonyms Selenoprotein P, selenoprotein P, plasma, 1, SELP, SeP, SEPP1
Calculated Molecular Weight43 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberNM_001085486.2
Gene Symbol Selenoprotein P
Gene ID (NCBI) 6414
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49908
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Selenoprotein P, also known as SEPP1, is expressed in the liver and heart and secreted into the plasma(PMID: 8421687). Selenoprotein P is implicated as an extracellular antioxidant involved in the transport of selenium to extra-hepatic tissues via apolipoprotein E receptor-2 (apoER2) and provides selenium to tissues such as brain and testis. In humans, it exists in plasma as two isoforms of approximately 50 and 60 kDa(PMID:19453253). Selenoprotein P is a glycosylated protein and deglycosylation of SEPP1 results in 45 kDa and 35 kDa isoforms(PMID: 25298198).

实验方案

Product Specific Protocols
WB protocol for Selenoprotein P antibody 30249-1-APDownload protocol
IHC protocol for Selenoprotein P antibody 30249-1-APDownload protocol
IF protocol for Selenoprotein P antibody 30249-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols