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Septin 5 Monoclonal antibody

Septin 5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E2A3

Cat no : 68075-1-Ig

Print datasheet

Synonyms

CDCREL, CDCREL 1, CDCREL1, H5, Peanut like protein 1, PNUTL1, SEPT5, septin 5, septin5

验证数据展示

  • WB analysis using 68075-1-Ig

    WB analysis using 68075-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68075-1-Ig (Septin 5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inpig brain tissue, LNCaP cells, Jurkat cells, K-562 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68075-1-Ig targets Septin 5 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Immunogen Septin 5 fusion protein Ag2205 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name septin 5
Synonyms CDCREL, CDCREL 1, CDCREL1, H5, Peanut like protein 1, PNUTL1, SEPT5, septin 5, septin5
Calculated Molecular Weight 369 aa, 43 kDa
Observed Molecular Weight40-43 kDa
GenBank Accession NumberBC025261
Gene Symbol Septin 5
Gene ID (NCBI) 5413
RRIDAB_2918813
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99719
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septin 5, a septin family member formerly called cell division control related protein 1 (CDCrel), is encoded in the 200 kb region and is abundantly expressed in the developing and adult brains of mice and humans. Although its constitutive deletion does not cause detectible structural or developmental abnormalities in the mouse brain, Septin 5 has been implicated in subtle neuronal and behavioral functions. Septin 5 is expressed in presynaptic terminals and exerts negative regulatory control over neurotransmitter release by binding to SNARE-protein, which has been implicated in neuropsychiatric disorders (PMID: 19240081). Septin 5 has 2 isoforms with the molecular mass of 39 and 43 kDa.

实验方案

Product Specific Protocols
WB protocol for Septin 5 antibody 68075-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols