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验证数据展示

  • IF Staining of MDCK using CL488-66542

    IF Staining of MDCK using CL488-66542

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite® Plus 488 Septin 7 antibody (CL488-66542, Clone: 1G12D1 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Septin 7 Monoclonal antibody

Septin 7 Monoclonal Antibody for IF/ICC
Cat No. CL488-66542

Print datasheet

CloneNo. 1G12D1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

septin7, SEPT7, SEPT 7, CDC3, CDC10 protein homolog

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMDCK cells
Planning an IHC experiment? We recommend our IHCeasy SEPT7 Ready-To-Use IHC Kit. SEPT7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66542-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66542 targets Septin 7 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen Septin 7 fusion protein Ag4309 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name septin 7
Synonyms septin7, SEPT7, SEPT 7, CDC3, CDC10 protein homolog
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 48-51 kDa
GenBank Accession NumberBC025987
Gene Symbol Septin 7
Gene ID (NCBI) 989
RRIDAB_3672913
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16181
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. Septin 7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Septin 7 antibody CL488-66542Download protocol
Standard Protocols
Click here to view our Standard Protocols