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验证数据展示

  • IF Staining of HeLa using 66188-1-Ig (same clone as 66188-1-PBS)

    IF Staining of HeLa using 66188-1-Ig (same clone as 66188-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Septin 8 antibody (66188-1-Ig, Clone: 4A12G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66188-1-Ig (same clone as 66188-1-PBS)

    WB analysis of HeLa using 66188-1-Ig (same clone as 66188-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66188-1-Ig (Septin 8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66188-1-Ig (same clone as 66188-1-PBS)

    IHC staining of mouse brain using 66188-1-Ig (same clone as 66188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66188-1-Ig (same clone as 66188-1-PBS)

    IHC staining of mouse brain using 66188-1-Ig (same clone as 66188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

Septin 8 Monoclonal antibody, PBS Only

Septin 8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66188-1-PBS

产品说明书

CloneNo. 4A12G9

宿主/亚型

Mouse / IgM

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

KIAA0202, SEP2, SEPT8, septin 8, septin8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

产品信息

66188-1-PBS targets Septin 8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Septin 8 fusion protein Ag19114 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 septin 8
别名 KIAA0202, SEP2, SEPT8, septin 8, septin8
计算分子量55.6 kDa
观测分子量 50-58 kDa
GenBank蛋白编号BC001329
基因名称 Septin 8
Gene ID (NCBI) 23176
RRIDAB_2881583
偶联类型 Unconjugated
形式 Liquid
纯化方式Thiophilic affinity chromatograph
UNIPROT IDQ92599
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Septin 8, also named as KIAA0202, is a member of the highly conserved septin family. Septins are membrane-associated GTPases which are involved in cytokinesis and cellular morphogenesis. Septin 8 is an interaction partner of Septin 4. It may play a role in platelet secretion.