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验证数据展示

  • IF Staining of HeLa using 66188-1-Ig

    IF Staining of HeLa using 66188-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Septin 8 antibody (66188-1-Ig, Clone: 4A12G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of HeLa using 66188-1-Ig

    WB analysis of HeLa using 66188-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66188-1-Ig (Septin 8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 66188-1-Ig

    IHC staining of mouse brain using 66188-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66188-1-Ig

    IHC staining of mouse brain using 66188-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Septin 8 Monoclonal antibody

Septin 8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66188-1-Ig

Print datasheet

CloneNo. 4A12G9

Host / Isotype

Mouse / IgM

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA0202, SEP2, SEPT8, septin 8, septin8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66188-1-Ig targets Septin 8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Septin 8 fusion protein Ag19114 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name septin 8
Synonyms KIAA0202, SEP2, SEPT8, septin 8, septin8
Calculated Molecular Weight55.6 kDa
Observed Molecular Weight 50-58 kDa
GenBank Accession NumberBC001329
Gene Symbol Septin 8
Gene ID (NCBI) 23176
RRIDAB_2881583
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDQ92599
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septin 8, also named as KIAA0202, is a member of the highly conserved septin family. Septins are membrane-associated GTPases which are involved in cytokinesis and cellular morphogenesis. Septin 8 is an interaction partner of Septin 4. It may play a role in platelet secretion.

实验方案

Product Specific Protocols
WB protocol for Septin 8 antibody 66188-1-IgDownload protocol
IHC protocol for Septin 8 antibody 66188-1-IgDownload protocol
IF protocol for Septin 8 antibody 66188-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols