验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells |
Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66188-1-Ig targets Septin 8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | Septin 8 fusion protein Ag19114 种属同源性预测 |
宿主/亚型 | Mouse / IgM |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | septin 8 |
别名 | KIAA0202, SEP2, SEPT8, septin 8, septin8 |
计算分子量 | 55.6 kDa |
观测分子量 | 50-58 kDa |
GenBank蛋白编号 | BC001329 |
基因名称 | Septin 8 |
Gene ID (NCBI) | 23176 |
RRID | AB_2881583 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Thiophilic affinity chromatograph |
UNIPROT ID | Q92599 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Septin 8, also named as KIAA0202, is a member of the highly conserved septin family. Septins are membrane-associated GTPases which are involved in cytokinesis and cellular morphogenesis. Septin 8 is an interaction partner of Septin 4. It may play a role in platelet secretion.
实验方案
Product Specific Protocols | |
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WB protocol for Septin 8 antibody 66188-1-Ig | Download protocol |
IHC protocol for Septin 8 antibody 66188-1-Ig | Download protocol |
IF protocol for Septin 8 antibody 66188-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |