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CoraLite® Plus 488-conjugated Septin 8 Monoclonal antibody

Septin 8 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4A12G9

Cat no : CL488-66188

Print datasheet

Synonyms

KIAA0202, SEP2, SEPT8, septin 8

验证数据展示

  • IF Staining of HeLa using CL488-66188

    IF Staining of HeLa using CL488-66188

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Septin 8 antibody (CL488-66188, Clone: 4A12G9 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

For other applications, we recommend the unconjugated version of this antibody, 66188-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66188 targets Septin 8 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Septin 8 fusion protein Ag19114 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name septin 8
Synonyms KIAA0202, SEP2, SEPT8, septin 8
Calculated Molecular Weight55.6 kDa
Observed Molecular Weight 50-58 kDa
GenBank Accession NumberBC001329
Gene Symbol Septin 8
Gene ID (NCBI) 23176
RRIDAB_3084219
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDQ92599
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septin 8, also named as KIAA0202, is a member of the highly conserved septin family. Septins are membrane-associated GTPases which are involved in cytokinesis and cellular morphogenesis. Septin 8 is an interaction partner of Septin 4. It may play a role in platelet secretion.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Septin 8 antibody CL488-66188Download protocol
Standard Protocols
Click here to view our Standard Protocols