• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of mouse placenta using 83980-3-RR (same clone as 83980-3-PBS)

    WB analysis of mouse placenta using 83980-3-RR (same clone as 83980-3-PBS)

    mouse placenta tissue was subjected to SDS PAGE followed by western blot with 83980-3-RR (Serpin E1/PAI-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83980-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00875-4

    Sandwich ELISA standard curve of MP00875-4

    Sandwich ELISA standard curve of MP00875-4, Mouse Serpin E1/PAI-1 Recombinant Matched Antibody Pair - PBS only. 83980-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1100. 83980-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

  • Sample test of MP00875-4

    Sample test of MP00875-4

    L-929 mouse fibroblast cells (1 x 10^6 cells/mL) were cultured for 3 days in MEM supplemented with 10% equine serum and stimulated with 10 μg/mL PHA. The cell culture supernatants was assayed for mouse Serpin E1/PAI-1 and measured 41,666.9 pg/mL.

  • Sample test of MP00875-4

    Sample test of MP00875-4

    Mouse heart tissue lyaste was measured. The mouse Serpin E1/PAI-1 concentration of detected samples was determined to be 1.46 ng/mL (based on a 4.6 mg/mL extract load).

  • Sample test of MP00875-4

    Sample test of MP00875-4

    Serum of six mice was measured. The mouse Serpin E1/PAI-1 concentration of detected samples was determined to be 16,187.9 pg/mL with a range of 6,614.7 - 24,758.6 pg/mL.

  • Cytometric bead array standard curve of MP00875-1

    Cytometric bead array standard curve of MP00875-1

    Cytometric bead array standard curve of MP00875-1, MOUSE Serpin E1/PAI-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83980-3-PBS. Detection antibody: 83980-2-PBS. Standard: Eg1100. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83980-3-RR

    Affinity and Kinetic Characterization of 83980-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83980-3-RR against Mouse Serpin E1/PAI-1 were performed. The affinity constant is 0.293 nM.

Mouse Serpin E1/PAI-1 Recombinant antibody, PBS Only (Capture/Detector)

Serpin E1/PAI-1 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83980-3-PBS

产品说明书

CloneNo. 241130G6

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

PAI-1, Serpine1, Serpin E1, Plasminogen activator inhibitor 1, PAI1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

83980-3-PBS targets Serpin E1/PAI-1 as part of a matched antibody pair:

MP00875-1: 83980-3-PBS capture and 83980-2-PBS detection (validated in Cytometric bead array)

MP00875-4: 83980-2-PBS capture and 83980-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 serine (or cysteine) peptidase inhibitor, clade E, member 1
别名 PAI-1, Serpine1, Serpin E1, Plasminogen activator inhibitor 1, PAI1
计算分子量45kDa
观测分子量45-50 kDa
基因名称 Serpin E1/PAI-1
Gene ID (NCBI) 18787
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP22777
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.