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Serpin E1/PAI-1 Recombinant antibody

Serpin E1/PAI-1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241130G6

Cat no : 83980-3-RR

Print datasheet

Synonyms

PAI-1, Serpine1, Serpin E1, Plasminogen activator inhibitor 1, PAI1

验证数据展示

  • WB analysis of mouse placenta using 83980-3-RR

    WB analysis of mouse placenta using 83980-3-RR

    mouse placenta tissue was subjected to SDS PAGE followed by western blot with 83980-3-RR (Serpin E1/PAI-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83980-3-RR

    Affinity and Kinetic Characterization of 83980-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83980-3-RR against Mouse Serpin E1/PAI-1 were performed. The affinity constant is 0.293 nM.

经过测试的应用

Positive WB detected inmouse placenta tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83980-3-RR targets Serpin E1/PAI-1 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name serine (or cysteine) peptidase inhibitor, clade E, member 1
Synonyms PAI-1, Serpine1, Serpin E1, Plasminogen activator inhibitor 1, PAI1
Calculated Molecular Weight45kDa
Observed Molecular Weight45-50 kDa
Gene Symbol Serpine1
Gene ID (NCBI) 18787
RRIDAB_3671554
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22777
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.

实验方案

Product Specific Protocols
WB protocol for Serpin E1/PAI-1 antibody 83980-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols