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  • Featured Product
  • KD/KO Validated

Sestrin 2 Monoclonal antibody, PBS Only

Sestrin 2 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1A1A4

Cat No : 66297-1-PBS

Print datasheet

Synonyms

HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2

验证数据展示

  • WB analysis of HEK-293T using 66297-1-Ig (same clone as 66297-1-PBS)

    WB analysis of HEK-293T using 66297-1-Ig (same clone as 66297-1-PBS)

    WB result of Sestrin 2 antibody (66297-1-Ig, 1:500) with si-control and si-Sestrin 2 transfected Hek293 cells. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

    WB analysis of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

    IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

    IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

    IF Staining of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Sestrin 2 antibody (66297-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

产品信息

66297-1-PBS targets Sestrin 2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, pig, rat
Immunogen Sestrin 2 fusion protein Ag20638 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sestrin 2
Synonyms HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC013304
Gene Symbol SESN2
Gene ID (NCBI) 83667
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP58004
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).