- Featured Product
- KD/KO Validated
Sestrin 2 Monoclonal antibody
Sestrin 2 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, rat and More (2)
Applications
IF/ICC, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
1A1A4
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293T |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66297-1-Ig targets Sestrin 2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, pig, rat samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC, CoIP |
| Tested Reactivity | human, pig, rat |
| Cited Reactivity | human, mouse, rat, pig, bovine |
| Immunogen | Sestrin 2 fusion protein Ag20638 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | sestrin 2 |
| Synonyms | HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2 |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC013304 |
| Gene Symbol | SESN2 |
| Gene ID (NCBI) | 83667 |
| RRID | AB_2881680 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P58004 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Sestrin 2 antibody 66297-1-Ig | Download protocol |
| IHC protocol for Sestrin 2 antibody 66297-1-Ig | Download protocol |
| IF protocol for Sestrin 2 antibody 66297-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Biol Toxicol Induction of Sestrin2 by pterostilbene suppresses ethanol-triggered hepatocyte senescence by degrading CCN1 via p62-dependent selective autophagy.
| ||
Osteoarthritis Cartilage Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism. | ||
J Nutr Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs. | ||
Cell Tissue Res Sestrin2 attenuates renal damage by regulating Hippo pathway in diabetic nephropathy. | ||
Am J Physiol Endocrinol Metab Prematurity blunts the insulin- and amino acid-induced stimulation of translation initiation and protein synthesis in skeletal muscle of neonatal pigs. | ||
Arch Pharm Res Sestrin2 induction contributes to anti-inflammatory responses and cell survival by globular adiponectin in macrophages
|
