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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Sestrin 2 Monoclonal antibody

Sestrin 2 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A1A4

Cat No : CL488-66297

Print datasheet

Synonyms

DKFZp761M0212, DKFZp761M02121, HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2

验证数据展示

  • IF Staining of HeLa using CL488-66297

    IF Staining of HeLa using CL488-66297

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Sestrin 2 antibody (CL488-66297, Clone: 1A1A4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-66297

    FC experiment of HeLa using CL488-66297

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Sestrin 2 (CL488-66297, Clone:1A1A4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66297-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66297 targets Sestrin 2 in IF, FC (Intra) applications and shows reactivity with human, pig, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, pig, rat
Immunogen Sestrin 2 fusion protein Ag20638 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sestrin 2
Synonyms DKFZp761M0212, DKFZp761M02121, HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC013304
Gene Symbol SESN2
Gene ID (NCBI) 83667
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP58004
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Sestrin 2 antibody CL488-66297Download protocol
FC protocol for CL Plus 488 Sestrin 2 antibody CL488-66297Download protocol
Standard Protocols
Click here to view our Standard Protocols