验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66297 targets Sestrin 2 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat, pig samples.
经测试应用 | IF/ICC, FC (Intra) Application Description |
经测试反应性 | human, rat, pig |
免疫原 | Sestrin 2 fusion protein Ag20638 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | sestrin 2 |
别名 | SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene |
计算分子量 | 54 kDa |
观测分子量 | 54 kDa |
GenBank蛋白编号 | BC013304 |
基因名称 | SESN2/Sestrin 2 |
Gene ID (NCBI) | 83667 |
RRID | AB_3084231 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P58004 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Sestrin 2 antibody CL488-66297 | Download protocol |
FC protocol for CL Plus 488 Sestrin 2 antibody CL488-66297 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |