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Sestrin 2 Recombinant antibody

Sestrin 2 Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

240034H3

Cat No : 83202-3-RR

Print datasheet

Synonyms

DKFZp761M0212, DKFZp761M02121, HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2

验证数据展示

  • WB analysis using 83202-3-RR

    WB analysis using 83202-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83202-3-RR (Sestrin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 83202-3-RR

    FC experiment of HEK-293 using 83202-3-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Sestrin 2 Recombinant antibody (83202-3-RR, Clone:240034H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive FC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83202-3-RR targets Sestrin 2 in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, FC, ELISA Application Description
Tested Reactivity Human
Immunogen Sestrin 2 fusion protein Ag1247 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sestrin 2
Synonyms DKFZp761M0212, DKFZp761M02121, HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2
Calculated Molecular Weight 54 kDa
Observed Molecular Weight54 kDa
GenBank Accession NumberBC013304
Gene Symbol SESN2
Gene ID (NCBI) 83667
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP58004
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SESN is a family of highly conserved, stress-inducible genes that can defend the cell against oxidative damage and oncogenic signaling. SESN2 ,which belongs to the SESN family, has been implicated as a tumor suppressor that can inhibit angiogenesis and promote autophagy, underscoring the importance of elucidating the molecular mechanism by which SESN2 regulates pathways of metabolism and survival.(PMID:22363791).

实验方案

Product Specific Protocols
WB protocol for Sestrin 2 antibody 83202-3-RRDownload protocol
FC protocol for Sestrin 2 antibody 83202-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols