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Sestrin 2 Recombinant antibody, PBS Only (Detector)

Sestrin 2 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

240034H3

Cat no : 83202-3-PBS

Print datasheet

Synonyms

SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene

验证数据展示

  • WB analysis using 83202-3-RR (same clone as 83202-3-PBS)

    WB analysis using 83202-3-RR (same clone as 83202-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83202-3-RR (Sestrin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83202-3-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 83202-3-RR (same clone as 83202-3-PBS)

    FC experiment of HEK-293 using 83202-3-RR (same clone as 83202-3-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Sestrin 2 Recombinant antibody (83202-3-RR, Clone:240034H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83202-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00278-1

    Cytometric bead array standard curve of MP00278-1

    Cytometric bead array standard curve ofMP00278-1, Sestrin2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83202-2-PBS. Detection antibody: 83202-3-PBS. Standard: Ag1247. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83202-3-RR

    Affinity and Kinetic Characterization of 83202-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83202-3-RR against Human Sestrin 2 were performed. The affinity constant is below 1 pM.

产品信息

83202-3-PBS targets Sestrin 2 as part of a matched antibody pair:

MP00278-1: 83202-2-PBS capture and 83202-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity Human
Immunogen Sestrin 2 fusion protein Ag1247 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sestrin 2
Synonyms SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene
Calculated Molecular Weight 54 kDa
GenBank Accession NumberBC013304
Gene Symbol SESN2/Sestrin 2
Gene ID (NCBI) 83667
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP58004
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C