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验证数据展示

  • IF Staining of mouse pancreas using 24496-1-AP

    IF Staining of mouse pancreas using 24496-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Somatostatin antibody (24496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), INS antibody (66198-1-Ig, Clone: 4B6A7, red).

  • IHC staining of mouse pancreas using 24496-1-AP

    IHC staining of mouse pancreas using 24496-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 24496-1-AP (Somatostatin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 24496-1-AP

    IHC staining of mouse pancreas using 24496-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 24496-1-AP (Somatostatin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Somatostatin (1-116aa) Polyclonal antibody

Somatostatin (1-116aa) Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 24496-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Synonyms

SMST, somatostatin, Somatostatin (1-116aa), SST, somatostatin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24496-1-AP targets Somatostatin (1-116aa) in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Somatostatin (1-116aa) fusion protein Ag21294 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name somatostatin
Synonyms SMST, somatostatin, Somatostatin (1-116aa), SST, somatostatin
Calculated Molecular Weight 13 kDa
GenBank Accession NumberBC032625
Gene Symbol Somatostatin
Gene ID (NCBI) 6750
RRIDAB_2918083
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61278
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Somatostatin is a peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones. Somatostatin is a useful marker of D-cells of pancreatic islet cells. Somatostatin inhibits ins and glucagon secretion. Somatostatin has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids. This antibody can recognize the full length and propeptide of Somatostatin.

实验方案

Product Specific Protocols
IHC protocol for Somatostatin (1-116aa) antibody 24496-1-APDownload protocol
IF protocol for Somatostatin (1-116aa) antibody 24496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols