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  • Featured Product
  • KD/KO Validated

Sortilin Polyclonal antibody, PBS Only

Sortilin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12369-1-PBS

Print datasheet

Synonyms

SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95

验证数据展示

  • WB analysis using 12369-1-AP (same clone as 12369-1-PBS)

    WB analysis using 12369-1-AP (same clone as 12369-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12369-1-AP (Sortilin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

    IHC staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SORT1 antibody included
  • IHC staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

    IHC staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SORT1 antibody included
  • IP experiment of rat brain using 12369-1-AP (same clone as 12369-1-PBS)

    IP experiment of rat brain using 12369-1-AP (same clone as 12369-1-PBS)

    IP result of anti-Sortilin (IP:12369-1-AP, 4ug; Detection:12369-1-AP 1:500) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

    IF Staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (12369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

    IF Staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (12369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • FC experiment of Neuro-2a using 12369-1-AP (same clone as 12369-1-PBS)

    FC experiment of Neuro-2a using 12369-1-AP (same clone as 12369-1-PBS)

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human Sortilin (12369-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12369-1-AP (same clone as 12369-1-PBS)

    IF Staining of HeLa using 12369-1-AP (same clone as 12369-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sortilin antibody (12369-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SORT1 Ready-To-Use IHC Kit. SORT1 primary antibody included.

产品信息

12369-1-PBS targets Sortilin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Sortilin fusion protein Ag2997 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sortilin 1
Synonyms SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95
Calculated Molecular Weight 831 aa, 92 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC023542
Gene Symbol Sortilin
Gene ID (NCBI) 6272
ENSEMBL Gene IDENSG00000134243
RRIDAB_2302242
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99523
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.