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验证数据展示

  • FC experiment of HepG2 using CL594-13791

    FC experiment of HepG2 using CL594-13791

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Spartin, SPG20 (CL594-13791) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL594-13791

    IF Staining of HepG2 using CL594-13791

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Spartin, SPG20 antibody (CL594-13791) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated Spartin/SPG20 Polyclonal antibody

Spartin/SPG20 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-13791

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra) and More (1)

别名

SPG20, KIAA0610, SPART, Spartin, SPG 20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 13791-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL594-13791 targets Spartin/SPG20 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 Spartin/SPG20 fusion protein Ag4815 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spastic paraplegia 20 (Troyer syndrome)
别名 SPG20, KIAA0610, SPART, Spartin, SPG 20
计算分子量 75 kDa, 85 kDa
GenBank蛋白编号BC047083
基因名称 Spartin
Gene ID (NCBI) 23111
RRIDAB_2919800
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N0X7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPG20 (spastic paraplegia 20) gene encodes a multifunctional Spartin protein. SPG20 protein is highly expressed in adipose tissue and may be implicated in endosomal trafficking and microtubule dynamics. SPG20 is mutated in Troyer syndrome, a hereditary spastic paraplegia defined by degeneration of upper motor neurons, recent study showed that regulation of SPG20 on mitochondrial calcium homeostasis may contribute to the pathophysiology of Troyer syndrome. Hypermethylation of SPG20 promoter, found in colorectal cancer patients, may play a role in cytokinesis arrest in colorectal tumorigenesis.

实验方案

Product Specific Protocols
IF protocol for CL594 Spartin/SPG20 antibody CL594-13791Download protocol
FC protocol for CL594 Spartin/SPG20 antibody CL594-13791Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Mol Cell

VPS4A is the selective receptor for lipophagy in mice and humans

Authors - Debajyoti Das
View Article