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验证数据展示

  • WB analysis of A549 using 60063-1-Ig (same clone as 60063-1-PBS)

    WB analysis of A549 using 60063-1-Ig (same clone as 60063-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

  • WB analysis using 60063-1-Ig (same clone as 60063-1-PBS)

    WB analysis using 60063-1-Ig (same clone as 60063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

  • WB analysis of T-47D using 60063-1-Ig (same clone as 60063-1-PBS)

    WB analysis of T-47D using 60063-1-Ig (same clone as 60063-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 60063-1-Ig (same clone as 60063-1-PBS)

    IHC staining of mouse kidney using 60063-1-Ig (same clone as 60063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STC2 antibody included
  • IHC staining of human breast cancer using 60063-1-Ig (same clone as 60063-1-PBS)

    IHC staining of human breast cancer using 60063-1-Ig (same clone as 60063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60063-1-Ig(Stanniocalcin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STC2 antibody included
  • IHC staining of human breast cancer using 60063-1-Ig (same clone as 60063-1-PBS)

    IHC staining of human breast cancer using 60063-1-Ig (same clone as 60063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60063-1-Ig(Stanniocalcin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STC2 antibody included
  • IF Staining of HeLa using 60063-1-Ig (same clone as 60063-1-PBS)

    IF Staining of HeLa using 60063-1-Ig (same clone as 60063-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Stanniocalcin 2 antibody (60063-1-Ig, Clone: 4H1E7 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60063-1-Ig (same clone as 60063-1-PBS)

    IF Staining of HeLa using 60063-1-Ig (same clone as 60063-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Stanniocalcin 2 antibody (60063-1-Ig, Clone: 4H1E7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50715-1

    Cytometric bead array standard curve of MP50715-1

    Cytometric bead array standard curve of MP50715-1, STC2/Stanniocalcin 2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60063-1-PBS. Detection antibody: 60063-2-PBS. Standard:Ag0359. Range: 0.391-100 ng/mL.

  • Featured Product
  • KD/KO Validated

Stanniocalcin 2 Monoclonal antibody, PBS Only (Capture)

Stanniocalcin 2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 60063-1-PBS
CloneNo. 4H1E7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

STC2, STC related protein, STC 2, Stanniocalcin-related protein, Stanniocalcin-2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy STC2 Ready-To-Use IHC Kit. STC2 primary antibody included.

产品信息

60063-1-PBS targets Stanniocalcin 2 as part of a matched antibody pair:

MP50715-1: 60063-1-PBS capture and 60063-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, pig
Immunogen Stanniocalcin 2 fusion protein Ag0359 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name stanniocalcin 2
Synonyms STC2, STC related protein, STC 2, Stanniocalcin-related protein, Stanniocalcin-2
Calculated Molecular Weight 33 kDa
Observed Molecular Weight44 kDa
GenBank Accession NumberBC000658
Gene Symbol Stanniocalcin 2
Gene ID (NCBI) 8614
RRIDAB_2197544
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO76061
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.