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验证数据展示

  • WB analysis of A549 using 60063-1-Ig (same clone as 60063-1-PBS)

    WB analysis of A549 using 60063-1-Ig (same clone as 60063-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

  • WB analysis using 60063-1-Ig (same clone as 60063-1-PBS)

    WB analysis using 60063-1-Ig (same clone as 60063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

  • WB analysis of T-47D using 60063-1-Ig (same clone as 60063-1-PBS)

    WB analysis of T-47D using 60063-1-Ig (same clone as 60063-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 60063-1-Ig (same clone as 60063-1-PBS)

    IHC staining of mouse kidney using 60063-1-Ig (same clone as 60063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STC2 antibody included
  • IHC staining of human breast cancer using 60063-1-Ig (same clone as 60063-1-PBS)

    IHC staining of human breast cancer using 60063-1-Ig (same clone as 60063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60063-1-Ig(Stanniocalcin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STC2 antibody included
  • IHC staining of human breast cancer using 60063-1-Ig (same clone as 60063-1-PBS)

    IHC staining of human breast cancer using 60063-1-Ig (same clone as 60063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60063-1-Ig(Stanniocalcin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STC2 antibody included
  • IF Staining of HeLa using 60063-1-Ig (same clone as 60063-1-PBS)

    IF Staining of HeLa using 60063-1-Ig (same clone as 60063-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Stanniocalcin 2 antibody (60063-1-Ig, Clone: 4H1E7 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60063-1-Ig (same clone as 60063-1-PBS)

    IF Staining of HeLa using 60063-1-Ig (same clone as 60063-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Stanniocalcin 2 antibody (60063-1-Ig, Clone: 4H1E7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50715-1

    Cytometric bead array standard curve of MP50715-1

    Cytometric bead array standard curve of MP50715-1, STC2/Stanniocalcin 2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60063-1-PBS. Detection antibody: 60063-2-PBS. Standard:Ag0359. Range: 0.391-100 ng/mL.

  • Featured Product
  • KD/KO验证

Stanniocalcin 2 Monoclonal antibody, PBS Only (Capture)

Stanniocalcin 2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 60063-1-PBS

产品说明书

CloneNo. 4H1E7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, pig

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

STC2, STC related protein, STC 2, Stanniocalcin-related protein, Stanniocalcin-2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy STC2 Ready-To-Use IHC Kit. STC2 primary antibody included.

产品信息

60063-1-PBS targets Stanniocalcin 2 as part of a matched antibody pair:

MP50715-1: 60063-1-PBS capture and 60063-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, pig
免疫原 Stanniocalcin 2 fusion protein Ag0359 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 stanniocalcin 2
别名 STC2, STC related protein, STC 2, Stanniocalcin-related protein, Stanniocalcin-2
计算分子量 33 kDa
观测分子量44 kDa
GenBank蛋白编号BC000658
基因名称 Stanniocalcin 2
Gene ID (NCBI) 8614
RRIDAB_2197544
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO76061
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.