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  • KD/KO Validated

Stanniocalcin 2 Monoclonal antibody

Stanniocalcin 2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4H1E7

Cat no : 60063-1-Ig

Print datasheet

Synonyms

stanniocalcin 2, Stanniocalcin related protein, STC 2, STC related protein, STC2, STCRP



经过测试的应用

Positive WB detected inA549 cells, hTERT-RPE1 cells, T-47D cells, HEK-293 cells, HT-29 cells, HCT 116 cells, HepG2 cells, PC-3 cells, pig brain tissue
Positive IHC detected inmouse kidney tissue, human breast cancer tissue, human kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STC2 Ready-To-Use IHC Kit. STC2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60063-1-Ig targets Stanniocalcin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse
Immunogen Stanniocalcin 2 fusion protein Ag0359 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name stanniocalcin 2
Synonyms stanniocalcin 2, Stanniocalcin related protein, STC 2, STC related protein, STC2, STCRP
Calculated Molecular Weight 33 kDa
Observed Molecular Weight44 kDa
GenBank Accession NumberBC000658
Gene Symbol Stanniocalcin 2/STC2
Gene ID (NCBI) 8614
RRIDAB_2197544
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO76061
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.

实验方案

Product Specific Protocols
WB protocol for Stanniocalcin 2 antibody 60063-1-IgDownload protocol
IHC protocol for Stanniocalcin 2 antibody 60063-1-IgDownload protocol
IF protocol for Stanniocalcin 2 antibody 60063-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Hepatocell Carcinoma

The Oncogenic and Diagnostic Potential of Stanniocalcin 2 in Hepatocellular Carcinoma.

Authors - Zhixian WuJin
humanIHC, WB

Biochem Biophys Res Commun

The novel prognostic risk factor STC2 can regulate the occurrence and progression of osteosarcoma via the glycolytic pathway.

Authors - Bo Yu
  • KD Validated
humanWB

Oncotarget

STC2 promotes head and neck squamous cell carcinoma metastasis through modulating the PI3K/AKT/Snail signaling.

Authors - Shuwen Yang
  • KD Validated
humanWB

Biomed Res Int

STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial-Mesenchymal Transition.

Authors - Chen Lin
  • KD Validated
humanIHC

BMC Med Genomics

Osteosarcoma transcriptome data exploration reveals STC2 as a novel risk indicator in disease progression

Authors - Ziyue Wang
humanWB

Arch Biochem Biophys

STC2 knockdown inhibits cell proliferation and glycolysis in hepatocellular carcinoma through promoting autophagy by PI3K/Akt/mTOR pathway

Authors - Ding Li