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验证数据展示

  • IF Staining of HeLa using CL594-60063

    IF Staining of HeLa using CL594-60063

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-60063 (Stanniocalcin 2 antibody) at dilution of 1:100.

  • FC experiment of HeLa using CL594-60063

    FC experiment of HeLa using CL594-60063

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human Stanniocalcin 2 (CL594-60063, Clone:4H1E7) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated Stanniocalcin 2 Monoclonal antibody

Stanniocalcin 2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-60063

产品说明书

CloneNo. 4H1E7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

STC2, STC related protein, STC 2, Stanniocalcin-related protein, Stanniocalcin-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STC2 Ready-To-Use IHC Kit. STC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60063-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60063 targets Stanniocalcin 2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Stanniocalcin 2 fusion protein Ag0359 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 stanniocalcin 2
别名 STC2, STC related protein, STC 2, Stanniocalcin-related protein, Stanniocalcin-2
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC000658
基因名称 Stanniocalcin 2
Gene ID (NCBI) 8614
RRIDAB_2883414
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO76061
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.

实验方案

Product Specific Protocols
IF protocol for CL594 Stanniocalcin 2 antibody CL594-60063Download protocol
Standard Protocols
Click here to view our Standard Protocols