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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Stanniocalcin 2 Monoclonal antibody

Stanniocalcin 2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4H1E7

Cat no : CL594-60063

Print datasheet

Synonyms

STC2, STC related protein, STC 2, Stanniocalcin-related protein, Stanniocalcin-2

验证数据展示

  • IF Staining of HeLa using CL594-60063

    IF Staining of HeLa using CL594-60063

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-60063 (Stanniocalcin 2 antibody) at dilution of 1:100.

  • FC experiment of HeLa using CL594-60063

    FC experiment of HeLa using CL594-60063

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human Stanniocalcin 2 (CL594-60063, Clone:4H1E7) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STC2 Ready-To-Use IHC Kit. STC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60063-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60063 targets Stanniocalcin 2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen Stanniocalcin 2 fusion protein Ag0359 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name stanniocalcin 2
Synonyms STC2, STC related protein, STC 2, Stanniocalcin-related protein, Stanniocalcin-2
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC000658
Gene Symbol Stanniocalcin 2/STC2
Gene ID (NCBI) 8614
RRIDAB_2883414
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO76061
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.

实验方案

Product Specific Protocols
IF protocol for CL594 Stanniocalcin 2 antibody CL594-60063Download protocol
Standard Protocols
Click here to view our Standard Protocols