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验证数据展示

  • IF Staining of HeLa using CL488-60063

    IF Staining of HeLa using CL488-60063

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Stanniocalcin 2 antibody (CL488-60063, Clone: 4H1E7 ) at dilution of 1:400.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Stanniocalcin 2 Monoclonal antibody

Stanniocalcin 2 Monoclonal Antibody for IF/ICC
Cat No. CL488-60063

产品说明书

CloneNo. 4H1E7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC

别名

stanniocalcin 2, Stanniocalcin related protein, STC 2, STC related protein, STC2, STCRP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STC2 Ready-To-Use IHC Kit. STC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60063-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60063 targets Stanniocalcin 2 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 Stanniocalcin 2 fusion protein Ag0359 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 stanniocalcin 2
别名 stanniocalcin 2, Stanniocalcin related protein, STC 2, STC related protein, STC2, STCRP
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC000658
基因名称 Stanniocalcin 2
Gene ID (NCBI) 8614
RRIDAB_2883094
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO76061
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Stanniocalcin 2 antibody CL488-60063Download protocol
Standard Protocols
Click here to view our Standard Protocols