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验证数据展示

  • WB analysis using 82559-1-RR (same clone as 82559-1-PBS)

    WB analysis using 82559-1-RR (same clone as 82559-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82559-1-RR (Stathmin 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82559-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 82559-1-RR (same clone as 82559-1-PBS)

    IHC staining of rat brain using 82559-1-RR (same clone as 82559-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82559-1-RR (Stathmin 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82559-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STMN1 antibody included
  • IHC staining of rat brain using 82559-1-RR (same clone as 82559-1-PBS)

    IHC staining of rat brain using 82559-1-RR (same clone as 82559-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82559-1-RR (Stathmin 1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82559-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STMN1 antibody included
  • IHC staining of human tonsillitis using 82559-1-RR (same clone as 82559-1-PBS)

    IHC staining of human tonsillitis using 82559-1-RR (same clone as 82559-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82559-1-RR (Stathmin 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82559-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STMN1 antibody included
  • IF Staining of mouse brain using 82559-1-RR (same clone as 82559-1-PBS)

    IF Staining of mouse brain using 82559-1-RR (same clone as 82559-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Stathmin 1 antibody (82559-1-RR, Clone: 2H6 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82559-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82559-1-RR (same clone as 82559-1-PBS)

    IF Staining of HeLa using 82559-1-RR (same clone as 82559-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Stathmin 1 antibody (82559-1-RR, Clone: 2H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82559-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 82559-1-RR (same clone as 82559-1-PBS)

    IF Staining of NIH/3T3 using 82559-1-RR (same clone as 82559-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using Stathmin 1 antibody (82559-1-RR, Clone: 2H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82559-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82559-1-RR

    Affinity and Kinetic Characterization of 82559-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82559-1-RR against Human Stathmin 1 were performed. The affinity constant is 7.57 nM.

Stathmin 1 Recombinant antibody, PBS Only

Stathmin 1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 82559-1-PBS

产品说明书

CloneNo. 2H6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

STMN1, Stathmin, OP18, Leukemia-associated phosphoprotein p18, LAP18

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy STMN1 Ready-To-Use IHC Kit. STMN1 primary antibody included.

产品信息

82559-1-PBS targets Stathmin 1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Stathmin 1 fusion protein Ag1633 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 stathmin 1/oncoprotein 18
别名 STMN1, Stathmin, OP18, Leukemia-associated phosphoprotein p18, LAP18
计算分子量 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC014353
基因名称 Stathmin 1
Gene ID (NCBI) 3925
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16949
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.