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验证数据展示

  • WB analysis using 82559-1-RR

    WB analysis using 82559-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82559-1-RR (Stathmin 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 82559-1-RR

    IHC staining of rat brain using 82559-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82559-1-RR (Stathmin 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STMN1 antibody included
  • IHC staining of rat brain using 82559-1-RR

    IHC staining of rat brain using 82559-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82559-1-RR (Stathmin 1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STMN1 antibody included
  • IHC staining of human tonsillitis using 82559-1-RR

    IHC staining of human tonsillitis using 82559-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82559-1-RR (Stathmin 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STMN1 antibody included
  • IF Staining of mouse brain using 82559-1-RR

    IF Staining of mouse brain using 82559-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Stathmin 1 antibody (82559-1-RR, Clone: 2H6 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 82559-1-RR

    IF Staining of HeLa using 82559-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Stathmin 1 antibody (82559-1-RR, Clone: 2H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of NIH/3T3 using 82559-1-RR

    IF Staining of NIH/3T3 using 82559-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using Stathmin 1 antibody (82559-1-RR, Clone: 2H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82559-1-RR

    Affinity and Kinetic Characterization of 82559-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82559-1-RR against Human Stathmin 1 were performed. The affinity constant is 7.57 nM.

Stathmin 1 Recombinant antibody

Stathmin 1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 82559-1-RR

产品说明书

CloneNo. 2H6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

STMN1, Stathmin, OP18, Leukemia-associated phosphoprotein p18, LAP18

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, Jurkat cells, PC-12 cells
Positive IHC detected inrat brain tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHeLa cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy STMN1 Ready-To-Use IHC Kit. STMN1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82559-1-RR targets Stathmin 1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Stathmin 1 fusion protein Ag1633 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 stathmin 1/oncoprotein 18
别名 STMN1, Stathmin, OP18, Leukemia-associated phosphoprotein p18, LAP18
计算分子量 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC014353
基因名称 Stathmin 1
Gene ID (NCBI) 3925
RRIDAB_3086489
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16949
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.

实验方案

Product Specific Protocols
WB protocol for Stathmin 1 antibody 82559-1-RRDownload protocol
IHC protocol for Stathmin 1 antibody 82559-1-RRDownload protocol
IF protocol for Stathmin 1 antibody 82559-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols